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KMID : 0900920020260030223
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Korean journal of Animal Reproduction
2002 Volume.26 No. 3 p.223 ~ p.228
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Effect of Antioxidants and Buthionine Sulfoximide on the Development of Porcine IVM/IVF Embryos
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Choi Young-Jin
Park Choon-Keun
Kim Choung-Ik
Cheong Hee-Tae
Park Dong-Heon
Jang Hyun-Yong
Park Jin-Ki
Yang Boo-Keun
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Abstract
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This study was carried out to examine the effects of antioxidants and buthionine sulfoximide(BSO) on the development of porcine in vitro maturation(IVM) and in vitro fertilization(IVF) oocytes. Cumulus cell free embryos derived from porcine IVM/IVF oocytes were cultured NCSU23 medium with antioxidants or BSO under an atmosphere of 5% CO_2 and 5% O_2at 38.5^{circ}C for 5~6 days. The embryos cultured in medium with BSO showed a significanthy(P<0.05) lower rates(8.4~15.7%) of the development to the morulae and blastocyst stage than control group(35.9%). When the embryos were cultured with NAC, ebselen, glutathione and BSO, the proportions of embryos beyond morulae and blastocysts were significantly(P<0.05) higher in medium with NAC(40.5%), ebselen(44.2%) and glutathione(36.0%) than BSO(10.9%). In conclusions, these results indicate that NAC, ebselen and glutathione as a antioxidants can increase the proportion of embryos that develop beyond morulae stage. BSO, intracellular glutathione inhibitors, is suppressed the development of porcine embryos derived from IVM/IVF.
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KEYWORD
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Buthionine sulfoximide, N-acetyl cysteine, Ebselen, Glutathione, Porcine embyros
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